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Technical Manual...

PureYieldTM RNA Midiprep System...

INSTRUCTIONS FOR USE OF PRODUCTS Z3740, Z3741 AND Z3742....

PRINTED IN USA. Revised 3/09...

Part# TM279...

PureYieldTM RNA Midiprep System...

All technical literature is available on the Internet at www.promega.com/tbs/ Please visit the web site to verify that you are using the most current version of this Technical Manual. Please contact Promega Technical Services if you have questions on use of this system. E-mail techserv@promega.com....

1. Description ..........................................................................................................2 2. Product Components and Storage Conditions ............................................4 3. Before You Begin ...............................................................................................6...

A. Protocol Overview................................................................................................6 B. Processing Capacity .............................................................................................7 C. Equipment Requirements and Solution Preparation......................................9...

4. Preparing Sample Lysates ..............................................................................11...

A. Preparing Lysates from Tissue Samples ........................................................12 B. Preparing Lysates from Cultured Cells ..........................................................13 C. Preparing Lysates from Gram-Positive (B. subtilis) and Gram-Negative (E. coli ) Bacteria .............................................................14 D. Preparing Lysates from Yeast...........................................................................15 E. Preparing Lysates from Plant Tissue...............................................................16 F. Preparing Lysates from Blood..........................................................................17 G. Cleanup of RNA Samples .................................................................................19...

5. Lysate Clearing (All Samples).......................................................................21 6. RNA Purification .............................................................................................23...

A. RNA Purification by Centrifugation (Spin)....................................................23 B. RNA Purification by Vacuum (Vac)................................................................24...

7. Determining RNA Yield and Qua...

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Complete Protocol media files resources protocols technical manuals pure yield rna midi prep system protocol

About Promega Corp.

Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high - value products for the life sciences. The Promega mission statement is:...

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